Phasing

Hi-C data plays a crucial role in genome phasing. By capturing long-range chromatin contacts, Hi-C provides linkage information between heterozygous variants that may be separated by megabases—well beyond the reach of standard short-read phasing methods. These 3D interaction signals can be used to group alleles into extended haplotype blocks, enabling accurate separation of maternal and paternal chromosomes, resolving complex or repetitive regions, and producing chromosome-scale, phased genomes.

Compatible Tools

Libraries generated by Dovetail Genomics kits, such as Omni-C and LinkPrep, are compatible with a wide range of haplotype phasing tools that utilize Hi-C data.

These include, but are not limited to, the following:

• HapCUT2

• FALCON-Phase

• DipAsm

• ALLHiC

• Diphase

• HapHiC

• HiFiasm

• C-Phasing